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Image Search Results
Journal: Frontiers in Molecular Biosciences
Article Title: Cooperation of Conical and Polyunsaturated Lipids to Regulate Initiation and Processing of Membrane Fusion
doi: 10.3389/fmolb.2021.763115
Figure Lengend Snippet: Adding DOPE in a phosphatidylcholine (PC) membrane or increasing the degree of unsaturation of the PC tails leads to an increase of the fusion speed. (A) Dequenching curves at 37 °C for different lipid compositions. For the sake of clarity, not all the intermediate curves are represented. However, all the data are presented in (B,C) . (B–C) Speed of fusion (in fraction of the total vesicles which undergo a fusion event in 1 minute) for different compositions on a semi-log scale (B) and on a linear scale with values normalized by that of pure POPC fusion speed (C) .
Article Snippet: DOPC (1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine),POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine), DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine), PDPC (1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine), NBD-DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) and
Techniques:
Journal: Frontiers in Molecular Biosciences
Article Title: Cooperation of Conical and Polyunsaturated Lipids to Regulate Initiation and Processing of Membrane Fusion
doi: 10.3389/fmolb.2021.763115
Figure Lengend Snippet: Lipid composition impacts the activation energy and the nucleation rate of fusion. (A) The variation of the initial spontaneous fusion speeds are plotted against the temperature (from 27 to 47°C) for DOPC, DOPC-25%DOPE, DOPC-50%DOPE, POPC, POPC-25%DOPE, POPC-50% DOPE, and PDPC. One fusion cycle corresponds to 100% of the vesicles of the sample which have undergone fusion. For the sake of clarity, the averages of the fusion speeds of the independent experiments are represented (error bars are standard errors on the mean) even though the activation energies values in (B) were deduced from independent fits. (B) The activation energy values and nucleation frequencies are represented for the different lipid compositions. Activation energies (empty bars) are determined through independent fits of 3–9 different experiments and the error bars correspond to the standard errors on the mean. Nucleation frequencies (filled bars) are determined from the average fit.
Article Snippet: DOPC (1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine),POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine), DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine), PDPC (1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine), NBD-DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) and
Techniques: Activation Assay
Journal: Frontiers in Molecular Biosciences
Article Title: Cooperation of Conical and Polyunsaturated Lipids to Regulate Initiation and Processing of Membrane Fusion
doi: 10.3389/fmolb.2021.763115
Figure Lengend Snippet: Simulations of defects for 0, 12.5, 25, and 50% DOPE. (A) Top view of a simulation snapshot from the DOPC:DOPE (50:50 mol:mol) system. Hydrophobic atoms (starting from the C2 till the end methyl of either sn-1 or sn-2) are represented in yellow van der Waals spheres, polar heads are in gray. The visible yellow atoms represent hydrophobic defects. (B) Evolution of the surface fraction occupied by defects in function of the fraction of DOPE lipids. It should be noted that these values may underestimate the actual values in our experiments, where the bilayers are actually curved, therefore increasing the inadequation between the geometry of the external DOPE lipids and that of the membrane. The experimentally measured nucleation rates (from ) for total fractions of DOPE of 0, 25, and 50%, corresponding to DOPE fractions on the outer leaflet of respectively 0, 10, and 27% (from ) are also presented and qualitatively exhibit similar variation.
Article Snippet: DOPC (1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine),POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine), DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine), PDPC (1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine), NBD-DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) and
Techniques: